Erhunter162

Fluorescence is detected using a Roper Scientific PI-MAX III Superblue ICCD camera equipped with a Cerco 100-mm UV lens. After 2 × 2 pixel binning, the images are 512 × 512 pixels with a pixel resolution of 92 µm. An EPICS areaDetector driver for the Roper Scientific detectors, which includes all cameras from Photometrics and most but not all cameras from Princeton Instruments. The driver is based upon the PVCAM library from Photometrics, and runs on Microsoft Windows and Linux. MDOTS were incubated with AO/PI reagents for 20 minutes in the darkness, and then images were taken with a Nikon Eclipse 80i fluorescence microscope equipped with z-stack (Prior) and a CoolSNAP CCD camera (Roper Scientific). Total area of each dye was measured to quantify live/dead cells. Vi VersArray System Manual Version 2.E vi Figure 20. Diagnostic Instruments Bottom Clamps for Different Microscopes.

Introduction¶

This plugin control a RoperScientific/Princeton camera under Windows and Linux, using the PVCAM (Photometrics Virtual Camera Access Method) libraries.

It is in production at SOLEIL under windows and it has been tested at Desy under Linux.Model used at SOLEIL: PI-MTE:2048B

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Prerequisite¶

The RoperScientific is connected to a specific computer with a PCI board. The Lima/RoperScientific client must run on this PC.

Initialisation and Capabilities¶

Implementing a new plugin for new detector is driven by the LIMA framework but the developer has some freedoms to choose which standard and specific features will be made available. This section is supposed to give you the correct information regarding how the camera is exported within the LIMA framework.

Camera initialisation¶

The camera will be initialized within the :cpp:RoperScientific::Camera object. The camera number (as an integer) should be given to the constructor. For example: 0.

Std capabilites¶

This plugin has been implemented in respect of the mandatory capabilites but with some limitations accordingto some programmer’s choices. We only provide here extra information for a better understandingof the capabilities for the RoperScientific camera.

• HwDetInfo

• Max image size is : 2048 * 2048

• 16 bit unsigned type is supported

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• HwSync

  Trigger type supported are:

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Optional capabilites¶

• HwBin:

• HwRoi

Specific control parameters¶

Some specific paramaters are available within the camera hardware interface. Those parameters should be used carefully and one should refer to the camera SDK (or user’s guide) documentation for a better understanding.

• getTemperature()

• set/getTemperatureSetPoint()

• set/getGain()

• set/getInternalAcqMode()

• set/getSpeedTableIndex()

Configuration¶

No Specific hardware configuration are needed

How to use¶

Here is the list of accessible fonctions to configure and use the RoperScientific detector:

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Code example in python:

Overview

WinSpec32 is a Shareware software in the category Miscellaneous developed by Roper Scientific.

The latest version of WinSpec32 is 2.5.5, released on 02/18/2008. It was initially added to our database on 11/19/2007.

WinSpec32 runs on the following operating systems: Windows.

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